Anti-VAMP2 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:RATVAMPB; RATVAMPIR; SYB2; Synaptobrevin 2; Vamp2l; Vesicle-associated membrane protein 2 (synaptobrevin 2);

Gene Synonyms:

Gene Full Name:

Gene Infomation:The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium. In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release. In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils and release of bioactive peptides from cardiac myocytes and juxtaglomerular cells.

Antigen:A synthesized peptide derived from human VAMP2

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:VAMP2 Antibody detects endogenous levels of total VAMP2

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:5000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

Mole Mass:16kDa

Location:Cell membrane;

Concentration:

Sequence Similarity:Contains 1 v-SNARE coiled-coil homology domain.

Gene Id:

SwissProt ID:P63027

Unigene:

Nucleotide Accession:

Tissue specificity:VAMP2 Antibody detects endogenous levels of total VAMP2

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium. In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.