Anti-Vimentin Mouse mAb

100ug (NP10275) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human

Gene Name:vim

Synonyms:HEL113; CTRCT30; VIM; vimentin.

Gene Synonyms:CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Vimentin protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4F8-A5-H5

Dilution:WB 1:1000,ICC 1:800

Mole Mass:57kDa

Location:Cytoplasm

Concentration:1.64mg/ml

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:7431

SwissProt ID:P08670

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.