Anti-Vimentin Mouse mAb (10E2)

100ug (NP11425) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IF,IHC

Purify method:Affinity purified

Species:Human,Rat,Mouse

Gene Name:VIM

Synonyms:FLJ36605, VIM, VIME

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:The Vimentin mouse monoclonal antibody candetectendogenous Vimentin proteins.

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:10E2-3B7-3D5

Dilution:WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 (Optimal dilutions should be determined by the end user)

Mole Mass:50-57kDa

Location:Cytoplasm, Intermediate filament

Concentration:

Sequence Similarity:

Gene Id:7431

SwissProt ID:P08670

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.