Anti-Vimentin Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:FLJ36605; VIM; VIME; Vimentin

Gene Synonyms:

Gene Full Name:

Gene Infomation:TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses. Members of the TRAF family share a common carboxy-terminal TRAF domain which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system.TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development.

Antigen:A synthesized peptide derived from human Vimentin

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Vimentin Antibody detects endogenous levels of total Vimentin

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:30

Mole Mass:54kDa

Location:Lipid droplet;

Concentration:

Sequence Similarity:Contains 2 TRAF-type zinc fingers.

Gene Id:

SwissProt ID:P08670

Unigene:

Nucleotide Accession:

Tissue specificity:Vimentin Antibody detects endogenous levels of total Vimentin

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.