Anti-ZAP-70 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Human

Gene Name:zap70

Synonyms:SRK;STD;TZK;STCD;ZAP-70.

Gene Synonyms:70 kDa zeta associated protein;70 kDa zeta-associated protein;EC 2.7.10.2;FLJ17670;FLJ17679;Selective T cell defect;SRK;STD;Syk related tyrosine kinase;Syk-related tyrosine kinase;Truncated ZAP kinase;Tyrosine protein kinase ZAP70;Tyrosine-protein kinase

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human ZAP-70 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of ZAP-70 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4B2-C1-H3

Dilution:WB 1:1000

Mole Mass:70kDa

Location:Cytoplasm.Cell membrane;Peripheral membrane protein.Note: In quiescent T-lymphocytes,it is cytoplasmic.Upon TCR activation,it is recruited at the plasma membrane by interacting with CD247/CD3Z.Colocalizes together with RHOH in the immunological synapse.RH

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily.Tyr protein kinase family.SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.

Gene Id:7535

SwissProt ID:P43403

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development.Contributes also to the development and activation of primary B-lymphocytes.When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation.Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK.Subsequently,ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn,a large number of signaling molecules are recruited and ultimately lead to lymphokine production,T-cell proliferation and differentiation.Furthermore,ZAP70 controls cytoskeleton modifications,adhesion and mobility of T-lymphocytes,thus ensuring correct delivery of effectors to the APC.ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2.Thus,ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface.During thymocyte development,ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative).Additionally,ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.