Anti-β-actin Mouse mAb (6F9)

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IHC,IF

Purify method:Affinity purified

Species:Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,Sheep

Gene Name:ACTB

Synonyms:actin, b-actin, beta-actin

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The beta-actin Mouse mAb can recognizeendogenous beta-actin proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6F9-1C10-4E8

Dilution:WB 1:5,000-10,000 IHC 1:200 IF 1:200 (Optimal dilutions should be determined by the end user)

Mole Mass:43kDa

Location:Cytoplasm, Cytoskeleton

Concentration:

Sequence Similarity:

Gene Id:60

SwissProt ID:P60709

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.