Anti-α-actinin Mouse mAb (1A5)

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:IHC

Purify method:Affinity purified

Species:Human,Rat,Mouse

Gene Name:ACTN1

Synonyms:actinin 1 smooth muscle, actinin alpha 1, ACTN 1, FLJ40884

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The alpha-actinin mouse monoclonal antibody can recognizeendogenous alpha-actinin proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:1A5-7D1-9H10

Dilution:IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)

Mole Mass:103kDa

Location:Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Concentration:

Sequence Similarity:

Gene Id:87

SwissProt ID:P12814

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.RegionsFeature keyPosition(s)Description

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.