Anti-beta Actin(HRP Conjugated)

Antibody type:Loading control antibody

Label:HRP

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB

Purify method:Affinity Chromatography

Species:Human,Mouse,Monkey,Rat,Hamster,Plants

Gene Name:

Synonyms:ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:A synthesized peptide derived from human beta Actin

Antigen Synonyms:

Clonality:

Source:

Reaction:beta Actin Antibody(HRP Conjugated) detects endogenous levels of total beta Actin

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:3000~1:10000

Mole Mass:42kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:P60709

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.