Anti-beta Tubulin (HRP conjugated) Rabbit pAb

Antibody type:Loading control antibody

Label:HRP

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:beta-4; CFEOM3A; MC1R; TBB3; TUBB3; TUBB4; Tubulin beta-3 chain; tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; tubulin, beta, 4,beta tubulin;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

Antigen:A synthesized peptide derived from human beta Tubulin

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:beta Tubulin Antibody (HRP conjugated) detects endogenous levels of total beta Tubulin

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:5000~1:20000

Mole Mass:50kDa

Location:Cytoplasm > Cytoskeleton;

Concentration:

Sequence Similarity:Belongs to the tubulin family.

Gene Id:

SwissProt ID:Q13509

Unigene:

Nucleotide Accession:

Tissue specificity:beta Tubulin Antibody (HRP conjugated) detects endogenous levels of total beta Tubulin

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.