Anti-eEF2 Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG2b
Host:Mouse
Application:WB,ICC
Purify method:Affinity purified
Species:Human
Gene Name:eef2
Synonyms:EF2; EF-2; EEF-2
Gene Synonyms:EEF2;Eef2;EF-2;EF2;EF2_HUMAN;Elongation factor 2;Eukaryotic translation elongation factor 2;Polypeptidyl tRNA translocase;SCA26.
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human eEF2 protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of eEF2 and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:4B3-G7-H5
Dilution:WB 1:5000,ICC 1:200
Mole Mass:95kDa
Location:Cytoplasm
Concentration:
Sequence Similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Gene Id:1938
SwissProt ID:P13639
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Background:Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.