Anti-eIF2α Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:eif2s1

Synonyms:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Gene Synonyms:DmelCG6376 ;Dmel_CG6376 ;drosE2F1 ;E(Sev-CycE)3A ;E(var)3-93E ;E2-promoter binding facto ;E2F 1 ;E2F transcription factor 1 ;E2F-1 ;E2f-PA ;E2f-PB ;E2f-PC ;E2F1 ;E2f1 E2F transcription factor 1 ;E2F1_HUMAN ;Evar(3)164 ;KIAA4009 ;l(3)07172 ;l(3)j3B1 ;l(3)

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human eIF2α protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of eIF2α and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2E4-F5-E7

Dilution:WB 1:1000,ICC 1:200

Mole Mass:38kDa

Location:Cytoplasmic granule.

Concentration:4.02mg/mL

Sequence Similarity:Belongs to the eIF-2-alpha family. Contains 1 S1 motif domain.

Gene Id:1965

SwissProt ID:P05198

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.