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Anti-eIF4E(Phospho-Ser209) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:eif4e

Synonyms:AUTS19;CBP;eIF 4E;eIF 4F 25 kDa subunit;EIF 4F;eIF-4E;eIF-4F 25 kDa subunit;eIF4E;EIF4E1;EIF4EL1;EIF4F;Eukaryotic translation initiation factor 4 E;Eukaryotic translation initiation factor 4E;Eukaryotic translation initiation factor 4E like 1;IF4E_HUMAN;Messanger RNA Cap Binding Protein eIF 4E;MGC111573;mRNA cap binding protein;mRNA cap-binding protein.

Gene Synonyms:CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1

Gene Full Name:

Gene Infomation:The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Antigen:The antiserum was produced against synthesized phosphopeptide derived from human eIF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:25

Location:Cytoplasm › P-body

Concentration:

Sequence Similarity:Belongs to the eukaryotic initiation factor 4E family.

Gene Id:1977

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of eIF4E and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.