Anti-eNOS Mouse mAb (2E8)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IHC

Purify method:Affinity purified

Species:Human,Rat,Mouse

Gene Name:NOS3

Synonyms:endothelial nitric oxidase synthase, NOS 3, NOS III, EC-NOS

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:The eNOS mouse monoclonal antibody can recognizeendogenous eNOS proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2E8-5A5-7B1

Dilution:WB 1:500-2,000 IHC 1：200-500 (Optimal dilutions should be determined by the end user)

Mole Mass:130kDa

Location:Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

Concentration:

Sequence Similarity:

Gene Id:4846

SwissProt ID:P29474

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.