Anti-iNOS Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP

Purify method:Affinity Chromatography

Species:Human,Mouse

Gene Name:

Synonyms:HEP-NOS; Hepatocyte NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS2;

Gene Synonyms:

Gene Full Name:

Gene Infomation:K14 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. Note: This description may include information from UniProtKB.

Antigen:A synthesized peptide derived from human iNOS

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:iNOS Antibody detects endogenous levels of total iNOS

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 ICC/IF 1:50~1:200 IP 1:50

Mole Mass:131kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:

SwissProt ID:P35228

Unigene:

Nucleotide Accession:

Tissue specificity:iNOS Antibody detects endogenous levels of total iNOS

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.