Anti-p16 INK Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:CDKN2A; CDKN2; MTS1; isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; P16INK4; p16INK4a; Multiple tumor suppressor 1;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome.Prior to posttranslational modification, Lysosome Associated Membrane Protein 2 (LAMP2) is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.

Antigen:A synthesized peptide derived from human p16 INK

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:p16 INK Antibody detects endogenous levels of total p16 INK

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:100

Mole Mass:17kDa

Location:Lysosome membrane > Single-pass type I membrane protein;

Concentration:

Sequence Similarity:Belongs to the LAMP family.

Gene Id:

SwissProt ID:P42771

Unigene:

Nucleotide Accession:

Tissue specificity:p16 INK Antibody detects endogenous levels of total p16 INK

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:p16-INK4A a cell-cycle regulatory protein that Interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6. Four alternatively spliced isoforms have been reported.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.