Anti-p27 KIP1 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:cdkn1b

Synonyms:KIP1; MEN4; CDKN4; MEN1B; P27KIP1; CDKN1B

Gene Synonyms:AA408329;AI843786;Cdki1b;CDKN 1B;CDKN 4;CDKN1B;CDKN1B;CDKN4;CDKN4;CDN1B_HUMAN;Cyclin Dependent Kinase Inhibitor 1B;Cyclin Dependent Kinase Inhibitor 1B;Cyclin dependent kinase inhibitor p27;Cyclin dependent kinase inhibitor p27;Cyclin-dependent kinase inh

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human p27 Kip1 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of p27 Kip1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5B4-E6-E11-A8

Dilution:WB 1:1000

Mole Mass:27kDa

Location:Nucleus. Cytoplasm. Endosome

Concentration:8.08mg/ml

Sequence Similarity:Belongs to the CDI family.

Gene Id:1027

SwissProt ID:P46527

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.