Anti-p70 S6 Kinase Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:EC 2.7.11.1; KS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase, Ribosomal protein S6 kinase 70kDa polypeptide 1; S6K1; kinase p70S6K; p70-S6K;

Gene Synonyms:

Gene Full Name:

Gene Infomation:p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression.Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo. Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229. Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor).

Antigen:A synthesized peptide derived from human p70 S6 Kinase

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:p70 S6 Kinase Antibody detects endogenous levels of total p70 S6 Kinase

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50 FC 1:100

Mole Mass:70kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:P23443

Unigene:

Nucleotide Accession:

Tissue specificity:p70 S6 Kinase Antibody detects endogenous levels of total p70 S6 Kinase

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression.Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo. Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.