Anti-phospho-MLKL (S358) Mouse(6F8) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Mouse

Application:IHC

Purify method:Affinity Chromatography

Species:Human

Gene Name:MLKL

Synonyms:MLKL

Gene Synonyms:hMLKL

Gene Full Name:

Gene Infomation:This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Antigen:Synthetic Peptide of phospho-MLKL (S358)

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:IHC::1:100-200

Mole Mass:54

Location:Cell membrane;

Concentration:

Sequence Similarity:Contains 1 protein kinase domain.

Gene Id:197259

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:phospho-MLKL (S358) protein detects endogenous levels of MLKL

Storage:

Buffer condition:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.