Anti-pyruvate dehydrogenase (lipoamide) α 1 Mouse mAb

100ug (NP10035) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:pdha1

Synonyms:PDHA;PHE1A;PDHCE1A.

Gene Synonyms:mitochondrial;ODPA_HUMAN;PDH;PDHA;PDHA1;PDHCE1A;PDHE1 A type I;PDHE1-A type I;PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1;Pyruvate dehydrogenase complex, E1 alpha polypeptide 1;Pyruvate Dehydrogenase E1 alpha;Pyruvate dehydrogenase E1 component subu

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of pyruvate dehydrogenase (lipoamide) alpha 1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3H2-F8-B5

Dilution:WB 1:1000,ICC 1:100

Mole Mass:43kDa

Location:Mitochondrion matrix

Concentration:

Sequence Similarity:

Gene Id:5160

SwissProt ID:P08559

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). Catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death.

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.