Anti-α tubulin Mouse mAb (Zebrafish Specific) (4C7)
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Antibody type:Loading control antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Zebrafish
Gene Name:TUBA4A
Synonyms:alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant protein expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The alpha-tubulin mouse monoclonal antibody candetect Zebrafishendogenous alpha-tubulin proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:4C7-3B1-10G4
Dilution:WB 1:5,000-10,000 (Optimal dilutions should be determined by the end user)
Mole Mass:52kDa
Location:Cytoplasm, Cytoskeleton, Microtubule
Concentration:
Sequence Similarity:
Gene Id:7277
SwissProt ID:P68363
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.SitesFeature keyPosition(s)Description
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.