AntiPhospho--MLKL (Ser358) Mouse mAb (10F6)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Phosphoralated

Isotype:IgG1

Host:Mouse

Application:IHC

Purify method:Affinity purified

Species:Human

Gene Name:MLKL

Synonyms:Mixed lineage kinase domain like protein, FLJ34389

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide conjugated to KLH.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The MLKL(Phospho Ser358) mouse monoclonal antibody can recognizeendogenous Phospho ser358 MLKL proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:10F6-8G10-2C6

Dilution:IHC 1:100-200 (Optimal dilutions should bedetermined by the end user)

Mole Mass:54kDa

Location:Cell membrane, Cytoplasm, Membrane

Concentration:

Sequence Similarity:

Gene Id:197259

SwissProt ID:Q8NB16

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.