Canine ALK1 / ACVRL1 Protein (Fc Tag)

ACVRL1

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Catalog Number	P70049-D02H
Organism Species	Canine
Host	Human Cells
Synonyms	ACVRL1
Molecular Weight	The recombinant canine ACVRL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 339 amino acids and has a predicted molecular mass of 37.9 kDa. The apparent molecular mass of the protein is approximately 43-47 kDa in SDS-PAGE under reducing conditions.
predicted N	Gly 22
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the canine ACVRL1(E2R174) (Met1-Gln119) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3-E1 cells. The ED₅₀ for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombinant human BMP-9.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Adhesion Molecules in Angiogenesis \|Extracellular Matrix \|Structures \|
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Reference	French Rendu-Osler network,et al. (2004) Molecular screening of ALK1/ACVRL1 and ENG genes in hereditary hemorrhagic telangiectasia in France. Hum Mutat. 23(4): 289-299. Simon M, et al. (2006) Association of a polymorphism of the ACVRL1 gene with sporadic arteriovenous malformations of the central nervous system. J Neurosurg. 104(6): 945-9. Argyriou L, et al. (2006) Novel mutations in the ENG and ACVRL1 genes causing hereditary hemorrhagic teleangiectasia. Int J Mol Med. 17(4):655-9.