Call Now

Canine CD40 / TNFRSF5 Protein

CD40, TNFRSF5

Catalog Number P70105-DCCH
Organism Species Canine
Host Human Cells
Synonyms CD40, TNFRSF5
Molecular Weight The recombinant canine CD40 is a disulfide-linked homodimer. The reduced monomer comprises 181 amino acids and has a predicted molecular mass of 19.9 kDa. The apparent molecular mass of the protein is approximately 28-32 kDa in SDS-PAGE under reducing conditions.
predicted N Glu 21
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the canine CD40 (Q7YRL5) (Met1-Ala194) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Bio-activity
Research Area Immunology |Innate Immunity |Cytokine |TNF Superfamily |Processes Regulated by TNF Superfamily Members |Regulation of T Cell Co-stimulation by TNF Superfamily Members |
Formulation Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Reference
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.