Canine Ephrin-A5 / EFNA5 Protein (Fc Tag)

EFNA5

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Catalog Number	P70072-D02H
Organism Species	Canine
Host	Human Cells
Synonyms	EFNA5
Molecular Weight	The recombinant canine EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 424 amino acids and has a predicted molecular mass of 48.2 kDa. The apparent molecular mass of the protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
predicted N	Gln 21
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the canine EFNA5 (XP_850582.2) (Met1-Asn203) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Immobilized human EPHA3-His (P11459-H08H) at 10 μg/ml (100 μl/well) can bind canine EFNA5-Fc, The EC50 of canine EFNA5-Fc is 11.5-26.7 ng/ml.
Research Area	Cell Biology |Cell Cycle |Cell Differentiation
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Reference	Frisén J, et al. (1998) Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system. Neuron. 20(2): 235-43. Feldheim DA, et al. (2000) Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping. Neuron. 25(3): 563-74. Wahl S, et al. (2000) Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase. J Cell Biol. 149(2): 263-70.