Canine IL-1 beta / IL1B Protein

IL-1

100ug (NPP1815) Please inquiry

Catalog Number	P70018-DNAE
Organism Species	Canine
Host	E. coli
Synonyms	IL-1
Molecular Weight	The recombinant canine IL1B consists of 153 amino acids and has a calculated molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.
predicted N	Met
SDS-PAGE
Purity	> 96 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of canine IL1B (Q28292) (Ala 115-Ser 266) was expressed, with an initial Met at the N-terminus.
Bio-activity	1. Measured by its ability to bind human IL1R1 in a functional ELISA. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is typically 2-12 pg/mL.
Research Area	Cardiovascular \|Atherosclerosis \|Vascular Inflammation \|Inflammatory mediators
Formulation	Lyophilized from sterile 20mM PB, 0.3M NaCl, pH 6.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Reference	Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16. Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42. Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.