Canine IL21 / Interleukin 21 Protein

IL-21

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Catalog Number	P70016-DNAE
Organism Species	Canine
Host	E. coli
Synonyms	IL-21
Molecular Weight	The recombinant canine IL21 consists of 130 amino acids and has a calculated molecular mass of 15 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 15KDa band as predicted.
predicted N	Met
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the canine IL21 (Q6L7I9) (His 18-Ser 146) was expressed, with an initial Met at the N-terminus.
Bio-activity	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Research Area	Immunology |Inflammation / Inflammatory Mediator |Inflammatory Cytokines & Chemoki and Receptors |Common gamma Chain Receptor Family
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34. Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10. Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.