Canine IL22RA1 / IL22R Protein (His Tag)

IL22RA1

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Catalog Number	P70083-D08H
Organism Species	Canine
Host	Human Cells
Synonyms	IL22RA1
Molecular Weight	The recombinant canine IL22RA1 comprises 223 amino acids and has a predicted molecular mass of 25.6 kDa. The apparent molecular mass of the protein is approximately 36 kDa in SDS-PAGE under reducing conditions.
predicted N	Ala 15
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the canine IL22RA1 (XP_855113.2) (Met1-The226) was expressed with a C-terminal polyhistidine tag.
Bio-activity	Immobilized canine IL22RA1-His at 10 μg/ml (100 μl/well) can bind biotinylated IL22 (P13059-HNAE), The EC₅₀ of biotinylated IL22 (P13059-HNAE) is 0.23-0.55 μg/ml.
Research Area	Cancer \|Signal transduction \|Protein Trafficking \|Vesicle Transport \|Adapters \|Transmembrane \|
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	IL-22R belongs to the type II cytokine receptor family. It contains 2 fibronectin type-III domains and is expressed in colon, liver, lung, pancreas and kidney. IL-22R also can be expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Overexpression of IL-22R can be detected in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients. IL-22R is a component of the receptor for IL20, IL22 and IL24. The component of IL-22R formed by IL22RA1 and IL10RB enables IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. IL-22R mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.
Reference	Xie MH, et al. (2000) Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 275(40):31335-9. Xu W, et al. (2001) A soluble class II cytokine receptor, IL-22RA2, is a naturally occurring IL-22 antagonist. Proc Natl Acad Sci. 98(17):9511-6. Wu PW, et al. (2008) IL-22R, IL-10R2, and IL-22BP binding sites are topologically juxtaposed on adjacent and overlapping surfaces of IL-22. J Mol Biol. 382(5):1168-83.