Canine IL33 / Interleukin-33 / NF-HEV Protein

DVS27

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Catalog Number	P70005-DNAE
Organism Species	Canine
Host	E. coli
Synonyms	DVS27
Molecular Weight	The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
predicted N	Met
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 (P70005-DNAE) at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc (P10105-H02H), The EC₅₀ of human IL1R4-Fc (P10105-H02H) is 0.25-0.66 μg/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with anti-CD3. The ED₅₀ for this effect is typically 10-40 ng/mL.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Cytokines / Chemokines in Angiogenesis \|Interleukins
Formulation	Lyophilized from sterile 5mM EDTA, PBS, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
Reference	Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8. Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.