Canine TNF-alpha / TNFA / TNFSF1A Protein
TNFA, Ctnf
- 100ug (NPP1705) Please inquiry
| Catalog Number | P70003-DNAE |
|---|---|
| Organism Species | Canine |
| Host | E. coli |
| Synonyms | TNFA, Ctnf |
| Molecular Weight | The recombinant canine TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 96 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the soluble form of canine TNFα (NP_001003244.4) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus. |
| Bio-activity | Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D . The ED50 for this effect is typically 0.2-1.2 ng/ml . |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Oncoprotein |Growth Factor & Receptor |Tumor Necrosis Factor (TNF) & Receptor |Tumor Necrosis Factor (TNF) | |
| Formulation | Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. |
| Reference |