Canine XCL1 Protein (His Tag)

XCL1

Catalog Number	P70096-D08B
Organism Species	Canine
Host	Baculovirus-Insect Cells
Synonyms	XCL1
Molecular Weight	The recombinant canine XCL1 comprises 98 amino acids and has a predicted molecular mass of 11.3 kDa. The apparent molecular mass of the protein is approximately 18-23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Glu 25
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the canine XCL1 (XP_547477.2) (Met1-Thr112) was expressed with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Inflammatory Cytokines & Chemoki and Receptors \|Chemokines and Receptors \|C Chemokines and Receptors
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Nguyen KD, et al. (2008) XCL1 enhances regulatory activities of CD4⁺ CD25(high) CD127(low/-) T cells in human allergic asthma. J Immunol. 181 (8): 5386-95. Dong C, et al. (2005) Glycosylated recombinant human XCL1 / lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 302 (1-2): 136-44. Zavala-Flores LM, et al. (2009) Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis. Biotechnol Lett. 31 (2): 215-20.