Cynomolgus ACVR2B / ACTRIIB Protein

ACVR2B

100ug (NPP1043) Please inquiry

Catalog Number	P90056-CCCH
Organism Species	Cynomolgus
Host	Human Cells
Synonyms	ACVR2B
Molecular Weight	The recombinant cynomolgus ACVR2B is a disulfide-linked homodimer. The reduced monomer comprises 123 amino acids and has a calculated molecular mass of 14.1 KDa.The apparent molecular mass of the protein is approximately 34 KDa in SDS-PAGE.
predicted N	Ser 28
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the cynomolgus ACVR2B (EHH16502.1) (Ser28-Thr143) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Bio-activity	Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 0.2-0.6 µg/mL in the presence of 10 ng/mL recombinant Activin A.
Research Area	Cancer \|Signal transduction \|Cytoskeleton / ECM \|Cytoskeletal Proteins \|Microfilaments \|Actin etc \|
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
Reference	Kosaki R, et al. (1999) Left-right axis malformations associated with mutations in ACVR2B, the gene for human activin receptor type IIB. Am J Med Genet. 82(1):70-6. Dupont S, et al. (2001) No evidence for linkage or for diabetes-associated mutations in the activin type 2B receptor gene (ACVR2B) in French patients with mature-onset diabetes of the young or type 2 diabetes. Diabetes 50(5):1219-21. Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18. Walsh S, et al. (2007) Activin-type II receptor B (ACVR2B) and follistatin haplotype associations with muscle mass and strength in humans. J Appl Physiol. 102(6):2142-8.