Cynomolgus B7-DC / PD-L2 / CD273 Protein (Fc Tag)
PDCD1LG2
- 100ug (NPP1570) Please inquiry
Catalog Number | P90249-C02H |
---|---|
Organism Species | Cynomolgus |
Host | Human Cells |
Synonyms | PDCD1LG2 |
Molecular Weight | The recombinant cynomolgus PDCD1LG2 is a disulfide-linked homodimer. The reduced monomer comprises 441 amino acids and has a calculated molecular mass of 49.6 KDa. The apparent molecular mass of the protein is approximately 66 KDa respectively in SDS-PAGE. |
predicted N | Leu 20 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the cynomolgus PDCD1LG2(A4GW30) (Met1-Pro219) was expressed with the Fc region of human IgG1 at the C-terminus. |
Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human PD1-His (P10377-H08H) at 10 μg/ml (100 μl/well) can bind Cynomolgus PDCD1LG2-Fc (P90249-C02H), The EC50 of Cynomolgus PDCD1LG2-Fc is 30.0-75.0 ng/ml. |
Research Area | Immunology |Innate Immunity |Monocytes/Macrophages |Co-stimulatory Molecules |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1. |
Reference |