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Cynomolgus CD32a / FCGR2A Protein (His & AVI Tag), Biotinylated

CD32a

Catalog Number P90015-C27H-B
Organism Species Cynomolgus
Host Human Cells
Synonyms CD32a
Molecular Weight The recombinant cynomolgus CD32a consists of 206 amino acids and has a calculated molecular mass of 22.9 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N Ala 30
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32a (AAL92096.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Bio-activity Labeling ratio of biotin to protein: 1.1
Research Area Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference