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Cynomolgus CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated

CD32b

Catalog Number P90014-C27H-B
Organism Species Cynomolgus
Host Human Cells
Synonyms CD32b
Molecular Weight The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N Ala 45
SDS-PAGE
Purity > 97 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Bio-activity Labeling ratio of biotin to protein: 1.1
Research Area Immunology |Innate Immunity |Granulocyte Markers
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference