Cynomolgus CD3D & CD3E Heterodimer Protein
CD3D & CD3E
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Catalog Number | PCT032-C0323H |
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Organism Species | Cynomolgus |
Host | Human Cells |
Synonyms | CD3D & CD3E |
Molecular Weight | The recombinant heterodimer of cynomolgus CD3E&CD3D comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of cyno CD3E&CD3D heterodimer is approximately 52 & 43 KDa respectively in SDS-PAGE. |
predicted N | Gln 22 & Phe 22 |
SDS-PAGE | |
Purity | (50.3+48.5) % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3E extracellular domain (Met 1-Asp 117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3D extracellular domain (Met 1-Ala 105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3E&CD3D heterodimer was purified. |
Bio-activity | |
Research Area | |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | |
Reference |