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Cynomolgus CD59 / CD59A / MAC-IP Protein (His Tag)

CD59

Catalog Number P90188-C08H
Organism Species Cynomolgus
Host Human Cells
Synonyms CD59
Molecular Weight The recombinant cynomolgus CD59 comprises 87 amino acids and has a calculated molecular mass of 10.2 KDa. The apparent molecular mass of it is approximately 18 and 14 KDa respectively in SDS-PAGE.
predicted N Leu 26
SDS-PAGE
Purity (77.8+20.8) % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the cynomolgus CD59 (G7PQF7) (Met1-Glu101) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area Cardiovascular |Atherosclerosis |Vascular Inflammation |Innate and adaptive immunity
Formulation Lyophilized from sterile PBS, PH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Reference
  • Fletcher CM. et al., 1994, Structure. 2: 185-99.
  • Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
  • Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
  • Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
  • Picariello G. et al., 2008, Proteomics 8: 3833-47.
  • Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.