Cynomolgus / Human TNFSF12 Protein (Fc Tag)

TNFSF12

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Catalog Number	P90094-C04H
Organism Species	Cynomolgus
Host	Human Cells
Synonyms	TNFSF12
Molecular Weight	The recombinant cynomolgus TNFSF12 is a disulfide-linked homodimer. The reduced monomer comprises 392 amino acids and has a calculated molecular mass of 43.8 KDa. The apparent molecular mass of it is approximately 34 and 47 KDa respectively in SDS-PAGE.
predicted N	Asp
SDS-PAGE
Purity	(72.2+25.9) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the cynomolgus TNFSF12 (F7HGN4) (Ser94-His249) was expressed with the Fc region of mouse IgG1 at the N-terminus. Cynomolgus and Human TNFSF12 sequences are identical.
Bio-activity	1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is typically 2-8 ng/mL. 2. Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A (P10431-H01H), The EC₅₀ of human Fc-TNFRSF12A (P10431-H01H) is 0.07-0.15 μg/ml.
Research Area	Cancer \|Signal transduction \|Other Related Intracellular Topics \|Regulation of Apoptosis by TNF Superfamily Members
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
Reference	Wiley SR, et al. (2004) TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cytokine Growth Factor Rev. 14(3-4):241-9. Campbell S, et al. (2006) The role of TWEAK/Fn14 in the pathogenesis of inflammation and systemic autoimmunity. Front Biosci. 9:2273-84. Lynch CN, et al. (1999) TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 274(13):8455-9.