Cynomolgus PDGFB / PDGF-BB Protein (Fc Tag)
PDGFB
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| Catalog Number | P90030-C04H |
|---|---|
| Organism Species | Cynomolgus |
| Host | Human Cells |
| Synonyms | PDGFB |
| Molecular Weight | The recombinant cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 amino acids and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE. |
| predicted N | Asp |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the cynomolgus PDGFB (EHH65851.1) (Ser82-Thr190) was expressed with the Fc region of mouse IgG1 at the N-terminus. |
| Bio-activity | 1. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His (P90215-C08H) at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml. |
| Research Area | Immunology |Cytokines & Growth Factors |Growth Factor & Receptor |Platelet-Derived Growth Factor (PDGF) & Receptor |Platelet-Derived Growth Factor (PDGF) |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |