Cynomolgus Thrombopoietin / THPO / TPO Protein (His Tag)
THPO, TPO
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| Catalog Number | P90004-C08H |
|---|---|
| Organism Species | Cynomolgus |
| Host | Human Cells |
| Synonyms | THPO, TPO |
| Molecular Weight | The recombinant cynomolgus THPO consists of 343 amino acids and has a calculated molecular mass of 36.8 kDa. The apparent molecular mass of the recombinant protein is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Ser 22 |
| SDS-PAGE |  |
| Purity | > 86 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the Cynomolgus (Macaca fascicularis) THPO (Met 1-Gly 353) precursor was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1-5 ng/ml. |
| Research Area | Cardiovascular |Blood |Coagulation |Coagulation Cascade |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action. |
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