Cynomolgus Tie2 / TEK Protein (Fc Tag)

TEK

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Catalog Number	P90292-C02H
Organism Species	Cynomolgus
Host	Human Cells
Synonyms	TEK
Molecular Weight	The recombinant cynomolgus TEK is a disulfide-linked homodimer. The reduced monomer comprises 964 amino acids and has a calculated molecular mass of 107.6 KDa.The apparent molecular mass of the protein is approximately 63 KDa respectively in SDS-PAGE.
predicted N	Ala 23
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the cynomolgus TEK (G7PS68) (Met1-Lys745) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized mouse S1h-3C-mANGPT2 (P50298-M07H) at 10 μg/ml (100 μl/well) can bind Cynomolgus TEK-Fc, The EC₅₀ of Cynomolgus TEK-Fc is 0.7-1.64 μg/ml.
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Inflammatory Cytokines & Chemoki and Receptors \|Angiopoietins and Angiopoietin-like Protein Families
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Reference	Jones N, et al. (1998) The Tek / Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108. Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27. Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK / TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.