Danio rerio (zebrafish) EFNB2A / Ephrin B2a Protein (His Tag)
EFNB2A, efnb2
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| Catalog Number | P63002-Z08H |
|---|---|
| Organism Species | Danio rerio (zebrafish) |
| Host | Human Cells |
| Synonyms | EFNB2A, efnb2 |
| Molecular Weight | The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | Leu 25 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc (P10235-H02H), The EC50 of human EphB4-Fc (P10235-H02H) is 15.5-36.3 ng/ml. |
| Research Area | Signaling |Signal Transduction |Growth Factor & Receptor |Ephrin & Eph Receptor |Ephrin |
| Formulation | Lyophilized from sterile PBS, PH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. |
| Reference |