Danio rerio (zebrafish) VEGF / VEGFA / VEGF165 Protein

VEGF165, vegf, vegfa, vegfaa

100ug (NPP1611) Please inquiry

Catalog Number	P63004-ZNAB
Organism Species	Danio rerio (zebrafish)
Host	Baculovirus-Insect Cells
Synonyms	VEGF165, vegf, vegfa, vegfaa
Molecular Weight	The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions.
predicted N	Ala 24
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.
Bio-activity	Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀ for this effect is typically 10-30 ng/mL.
Research Area	Cardiovascular \|Angiogenesis \|Growth Factor & Receptor \|Vascular Endothelial Growth Factor (VEGF) & Receptor \|Vascular Endothelial Growth Factor (VEGF)
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
Reference	Woolard J. et al. (2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res. 64(21): 7822-7835. Jia SF, et al. (2008) VEGF165 is necessary to the metastatic potential of Fas(-) osteosarcoma cells but will not rescue the Fas(+) cells. J Exp Ther Oncol. 7(2): 89-97. Cimpean AM, et al. (2008) Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma. Rom J Morphol Embryol. 49(3): 303-8. Hamdollah Zadeh MA, et al. (2008) VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6. Microcirculation. 15(7): 605-14. Eisenach PA, et al. (2010) MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src. J Cell Sci. 123(Pt 23):4182-4193. Claesson-Welsh L (2010) Gremlin: vexing VEGF receptor agonist. Blood. 116(18):3386-7.