Discosoma sp. orange / red fluorescent protein / OFP / RFP OFPSparkTM (His Tag)
OFP
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| Catalog Number | P69002-S08E |
|---|---|
| Organism Species | Discosoma sp |
| Host | E. coli |
| Synonyms | OFP |
| Molecular Weight | The recombinant Discosoma sp. OFPSparkTM consisting of 231 amino acids and has a calculated molecular mass of 26.4 kDa. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 99 % as determined by SDS-PAGE |
| Protein Construction | OFPSparkTM is a red (orange) fluorescent protein derived from DsRed(similarity is 83%). OFPSparkTM was expressed with a polyhistide tag at the C-terminus(Patent 201510003374.3). |
| Bio-activity | OFPSparkTM expression vector transfected 293 H cells transiently. After 48 h, the strong orange fluorescent signals can be detected under the excitation channel of 503.5~547.5 nm, and the strong red fluorescent signals can be detected under the excitation channel of 532.5~587.5 nm. |
| Research Area | |
| Formulation | Lyophilized from sterile Tris, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications. |
| Reference |