Escherichia coli ssPA / Stringent starvation protein A Protein (His Tag)

pog,ssp

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Catalog Number	P13098-E07E
Organism Species	E. coli
Host	E. coli
Synonyms	pog,ssp
Molecular Weight	The recombinant E.Coli ssPA consisting of 228 amino acids and has a calculated molecular mass of 26.4 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the E.Coli ssPA (P0ACA3) (Met 1-Ser 212) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Stringent starvation protein A (ssPA), a member of the GST superfamily, is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. It indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. SspA is known to be implicated in survival during nutrient starvation and prolonged stationary phase. Recently, SspA was shown to play an important role in the stationary phase-induced stress response including acid tolerance by down-regulating the level of the global regulator H-NS, which negatively regulates multiple stress defense systems.
Reference	Hansen AM, et al. (2003) Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol Microbiol. 48(6): 1621-31. Hommais F, et al. (2001) Large-scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid-associated protein, H-NS. Mol Microbiol. 40(1): 20-36. Atlung T, et al. (1997) H-NS: a modulator of environmentally regulated gene expression. Mol Microbiol. 24(1): 7-17. Williams MD, et al. (1994) Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation. Mol Microbiol. 11(6): 1029-43.