Call Now

Hepatitis C virus (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)

NS3

Catalog Number P40286-V09E
Organism Species HCV
Host E. coli
Synonyms NS3
Molecular Weight The recombinant Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 consists 335 amino acids and predicts a molecular mass of 37.9 kDa.
predicted N Met
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE.
Protein Construction A DNA sequence encoding the Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 (NP_803144.1) (Thr1356-Thr1459) was expressed with a GST tag at the N-terminus.
Bio-activity
Research Area Cancer |Invasion microenvironment |Adhesion molecule |Cell adhesion |Cadherins
Formulation Lyophilized from sterile 50 mM Tris, 500 mM NaCl.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.
Reference
  • Pomerantz J.L., et al.,(1999), NF-kB activation by a signaling complex containing TRAF2, TANK, and TBK1, a novel IKK-related kinase. EMBO J. 18:6694-6704.
  • Tojima Y., et al., (2000), NAK is an IkappaB kinase-activating kinase.Nature 404:778-782.
  • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.