Human 14-3-3 beta / YWHAB Protein
GW128,HEL-S-1,HS1,KCIP-1,YWHAA
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| Catalog Number | P10843-HNCE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | GW128,HEL-S-1,HS1,KCIP-1,YWHAA |
| Molecular Weight | The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 93 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus. |
| Bio-activity | |
| Research Area | Developmental Biology |Metabolism |Pathways and Processes |Metabolism processes |Apoptosis |Intracellular | |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation. |
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