Human ALDH1 / ALDH1A1 / ALDC Protein (His Tag)
ALDC,ALDH-E1,ALDH1,ALDH11,HEL-9,HEL-S-53e,HEL12,PUMB1,RALDH1
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| Catalog Number | P11388-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | ALDC,ALDH-E1,ALDH1,ALDH11,HEL-9,HEL-S-53e,HEL12,PUMB1,RALDH1 |
| Molecular Weight | The recombinant human ALDH1A1comprises 507 amino acids and has a predicted molecular mass of 41.5 kDa. It migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human ALDH1A1 (NP_000680.2) (Ser 2-Ser 501) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Cell Biology |Cell Cycle |Oxidative Stress |Additional Reduction/Oxidation (Redox) Enzymes |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), also known as Aldehyde dehydrogenase 1 (ALDH1), or Retinaldehyde Dehydrogenase 1 (RALDH1), is an enzyme that is expressed at high levels in stem cells and that has been suggested to regulate stem cell function. The retinaldehyde dehydrogenase (RALDH) subfamily of ALDHs, composed of ALDH1A1, ALDH1A2, ALDH1A3, and ALDH8A1, regulate development by catalyzing retinoic acid biosynthesis. The ALDH1A1 protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. Increased ALDH1A1 activity has been found in the stem cell populations of leukemia and some solid tumors. In tumor specimens, increased ALDH1A1 immunopositivity was found not only in secretory type cancer epithelial cells but also in neuroendocrine tumor populations. ALDH1 has been identified as a reliable marker of breast cancer stem cells. ALDH1 expression in primary cancer is an independent prognostic factor in node-positive breast cancer patients. ALDH1A1 plays a key role in normal hematopoiesis, and as a TLX1 transcriptional target, ALDH1A1 may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth. |
| Reference |