Human ALDH3A1 Protein (His Tag)

ALDH3,ALDHIII

Catalog Number	P12523-H07B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	ALDH3,ALDHIII
Molecular Weight	The recombinant human ALDH3A1 consists of 472 amino acids and has a calculated molecular mass of 52.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human ALDH3A1 (AAH04370.1) (Met 1-His 453) was fused with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Signaling \|Signal Transduction \|Metabolism \|Pathways and Processes \|Redox metabolism \|Oxidative stress \|
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Pappa A, et al. (2003) Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea. Biochem J. 376(3): 615-23. Estey T, et al. (2007) ALDH3A1: a corneal crystallin with diverse functions. Experimental Eye Research. 84(1):3-12. Pappa A, et al. (2003) Aldh3a1 protects human corneal epithelial cells from ultraviolet- and 4-hydroxy-2-nonenal-induced oxidative damage. Free Radical Biology and Medicine. 34(9):1178-89.