Human ALK4 / ACVR1B Protein (His Tag)
ACTRIB,ACVRLK4,ALK4,SKR2
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| Catalog Number | P10583-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | ACTRIB,ACVRLK4,ALK4,SKR2 |
| Molecular Weight | The recombinant human ACVR1B consists of 114 amino acids and has a predicted molecular mass of 13 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh ACVR1B is approximately 18-20 kDa due to glycosylation. |
| predicted N | Ser 24 |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human ACVR1B (NP_004293.1) extracellular domain (Met 1-Glu 126) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human TDGF1 at 2 μg/ml (100 μl/well) can bind human ALK-4 with a linear range of 0.032-4 μg/ml. |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Oncoprotein |Growth Factor & Receptor |Transforming Growth Factor Beta (TGF-beta) Superfamily |TGF-beta Superfamily Receptors | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination. |
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