Human APEX1 / AP / APEx / Ref-1 Protein (His Tag)
APE,APE1,APEN,APEX,APX,HAP1,REF1
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| Catalog Number | P12367-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | APE,APE1,APEN,APEX,APX,HAP1,REF1 |
| Molecular Weight | The recombinant human APEX1 consisting of 328 amino acids and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Cancer |Cell cycle |DNA Damage & Repair |Base Excision Repair |
| Formulation | Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation. |
| Reference |